![]() Red cell phenotype of the patient showed the absence of the M antigen. The antibody persisted after the serum was treated with DTT suggesting the presence of an IgG component along with IgM. In order to determine the immunoglobulin class of the antibody, treatment of the sera with dithiothreitol (DTT) was done. Antibody identification performed using as 11-cell panel from Immucor was suggestive of anti-M. Antibody screening was then performed with a commercially available three-cell panel ( Immuco r) and the antibody was found to be positive with one example of M-positive RBC and negative with two examples of M-negative RBCs. A direct antiglobulin test (DAT) was performed using polyspecific antiglobulin reagents (anti IgG and C3d) and was found to be negative. The blood grouping was repeated, and we found the same reaction. The ABO forward grouping of the patient was group B while the reverse grouping was group O, by tube technique. Case 1Īn 11-year-old female child with a diagnosis of hepatic disease was admitted in the hospital. 1–7 Here, we report for the first time in Iran, three cases with discrepancy in ABO blood grouping due to anti-M. ![]() In these cases, anti-M capability to react at 37 ☌ and in anti-human globulin (AHG) phase make it clinically significant. However, there are few studies that have reported anti-M as a mixture of IgG and IgM antibodies. This antibody is the most reactive at temperatures below 37 ☌, with an optimum temperature of 4 ☌ and is considered to be clinically insignificant. Anti- M is a naturally occurring antibody of the MNS blood group system. ![]()
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